Analytical Modeling of High Performance Reconfigurable Computers: Prediction and Analysis of System Performance.

The use of a network of shared, heterogeneous workstations each harboring a Reconfigurable Computing (RC) system offers high performance users an inexpensive platform for a wide range of computationally demanding problems. However, effectively using the full potential of these systems can be challenging without the knowledge of the system’s performance characteristics. While some performance models exist for shared, heterogeneous workstations, none thus far account for the addition of Reconfigurable Computing systems. This dissertation develops and validates an analytic performance modeling methodology for a class of fork-join algorithms executing on a High Performance Reconfigurable Computing (HPRC) platform. The model includes the effects of the reconfigurable device, application load imbalance, background user load, basic message passing communication, and processor heterogeneity. Three fork-join class of applications, a Boolean Satisfiability Solver, a Matrix-Vector Multiplication algorithm, and an Advanced Encryption Standard algorithm are used to validate the model with homogeneous and simulated heterogeneous workstations. A synthetic load is used to validate the model under various loading conditions including simulating heterogeneity by making some workstations appear slower than others by the use of background loading. The performance modeling methodology proves to be accurate in characterizing the effects of reconfigurable devices, application load imbalance, background user load and heterogeneity for applications running on shared, homogeneous and heterogeneous HPRC resources. The model error in all cases was found to be less than five percent for application runtimes greater than thirty seconds and less than fifteen percent for runtimes less than thirty seconds. The performance modeling methodology enables us to characterize applications running on shared HPRC resources. Cost functions are used to impose system usage policies and the results of vii the modeling methodology are utilized to find the optimal (or near-optimal) set of workstations to use for a given application. The usage policies investigated include determining the computational costs for the workstations and balancing the priority of the background user load with the parallel application. The applications studied fall within the Master-Worker paradigm and are well suited for a grid computing approach. A method for using NetSolve, a grid middleware, with the model and cost functions is introduced whereby users can produce optimal workstation sets and schedules for Master-Worker applications running on shared HPRC resources

After Dothard: Female Correctional Workers and the Challenge to Employment Law

This article examines a profession where women have made great strides - corrections. Using an equality framework, corrections and other non-traditional professions were the first target of the feminist movement in the 1970s. By and large, feminists were successful in creating greater porosity for women in law enforcement, emergency services, corrections, and the military. While women have entered these traditionally masculine spaces, they still suffer from an achievement gap. They are still underrepresented in leadership positions and marginalized in these settings; are still the targets of discrimination based on race, gender, and perceived sexual orientation; and are less likely than men to hold these positions and be married. Women\u27s entry into correctional spaces has had several unintended consequences. First, it has complicated the experiences of other marginalized groups in those institutions. In particular, women\u27s progress in correctional institutions has increased female inmates\u27 exposure to supervision by male staff, which places them at greater risk for sexual victimization. Second, it has diminished privacy of both male and female inmates in custodial settings. Third, it has resulted in female correctional employees\u27 disproportionate involvement in prohibited intimate contact with male inmates and youth in custody. These sexual interactions have resulted variously in termination, resignation, prosecution, procreation, and litigation; complicating feminist theories of power, consent, and equality. Finally, it has complicated key employment law jurisprudence. This article examines three areas of law and how female correctional staff\u27s roles have complicated those doctrines: (1) privacy for inmates under the Fourth and Eighth Amendments; (2) the bona fide occupational qualification ( BFOQ ) doctrine; and (3) sexual harassment under Title VII

The Sea Lamprey Meiotic Map Improves Resolution of Ancient Vertebrate Genome Duplications

It is generally accepted that many genes present in vertebrate genomes owe their origin to two whole-genome duplications that occurred deep in the ancestry of the vertebrate lineage. However, details regarding the timing and outcome of these duplications are not well resolved. We present high-density meiotic and comparative genomic maps for the sea lamprey (Petromyzon marinus), a representative of an ancient lineage that diverged from all other vertebrates ~550 million years ago. Linkage analyses yielded a total of 95 linkage groups, similar to the estimated number of germline chromosomes (1n ~ 99), spanning a total of 5570.25 cM. Comparative mapping data yield strong support for the hypothesis that a single whole-genome duplication occurred in the basal vertebrate lineage, but do not strongly support a hypothetical second event. Rather, these comparative maps reveal several evolutionarily independent segmental duplications occurring over the last 600+ million years of chordate evolution. This refined history of vertebrate genome duplication should permit more precise investigations of vertebrate evolution

Discovery of Sex-Specific Regions in a Salamander Genome

Biological Aspects: Salamander (Ambystoma mexicanum) has a gigantic genome: ~32,000,000,000 bases (10X of size of human genome) Sex is determined by a pair of morphologically identical chromosomes: ZZ in male ZW in female Object: Find (if there are any) genomic differences between chromosomes W and Z Workflow: Sequencing and de novo assembly of the reference salamander genome Alignment of short sequences from male and female genomes to the reference Coverage analysi

In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

<p>Abstract</p> <p>Background</p> <p>Rhabdoid tumors (RTs) are aggressive pediatric malignancies with poor prognosis. N-(4-hydroxy phenyl) retinamide (4-HPR or fenretinide) is a potential chemotherapeutic for RTs with activity correlated to its ability to down-modulate Cyclin D1. Previously, we synthesized novel halogen-substituted and peptidomimetic-derivatives of 4-HPR that retained activity in MON RT cells. Here we analyzed the effect of 4-HPR in inhibiting the growth of several RT, glioma, and breast cancer cell lines and tested their effect on cell cycle, apoptosis and Cyclin D1 expression.</p> <p>Methods</p> <p>Effect of compounds on RT cell cycle profiles, and cell death were assessed by MTS cell survival assays and FACS analysis. The effects of treatment on Cyclin D1 expression were determined by immunoblotting. The efficacy of these compounds on glioma and breast cancer cell lines was also determined using MTS assays.</p> <p>Results</p> <p>Low micromolar concentrations of 4-HPR derivatives inhibited cell survival of all RT cells tested. The 4-HPR derivatives altered RT cell cycle profiles and induced high levels of cell death that was correlated with their potency. ATRA exhibited high IC₅₀values in all cell lines tested and did not cause cell death. In MON RT cells, the iodo-substituted compounds were more active than 4-HPR in inducing cell cycle arrest and apoptosis. Additionally, the activity of the compounds correlated with their ability to down-modulate Cyclin D1: while active compounds reduced Cyclin D1 levels, inactive ATRA did not. In glioma and breast cancer cell lines, 4-HPR and 4-HPR derivatives showed variable efficacy.</p> <p>Conclusions</p> <p>Here we demonstrate, for the first time, that the inhibitory activities of novel halogen-substituted and peptidomimetic derivatives of 4-HPR are correlated to their ability to induce cell death and down-modulate Cyclin D1. These 4-HPR derivatives showed varied potencies in breast cancer and glioma cell lines. These data indicate that further studies are warranted on these derivatives of 4-HPR due to their low IC₅₀s in RT cells. These derivatives are of general interest, as conjugation of halogen radioisotopes such as ¹⁸F, ¹²⁴I, or ¹³¹I to 4-HPR will allow us to combine chemotherapy and radiotherapy with a single drug, and to perform PET/SPECT imaging studies in the future.</p

Evolving a more Active Telomerase Enzyme in Saccharomyces Cerevisiae

https://scholarworks.moreheadstate.edu/student_scholarship_posters/1259/thumbnail.jp

Injury profile suffered by targets of antipersonnel improvised explosive devices: prospective cohort study

Objective To describe pattern 1 injuries caused by the antipersonnel improvised explosive device (AP-IED) in comparison to those previously described for antipersonnel mines (APM). Design Prospective cohort study of 100 consecutive pedestrian victims of an AP-IED, with traumatic amputation without regard for gender, nationality or military status. Setting Multinational Medical Unit at Kandahar Air Field, Afghanistan. Participants One hundred consecutive patients, all male, 6–44 years old. Main outcome measures The details of injuries were recorded to describe the pattern and characterise the injuries suffered by the target of AP-IEDs. The level of amputation, the level of soft tissue injury, the fracture pattern (including pelvic fractures) as well as perineal, gluteal, genital and other injuries were recorded. Results Victims of AP-IED were more likely, compared with APM victims, to have multiple amputations (70.0% vs 10.4%; p\u3c0.001) or genital injury (26% vs 13%; p=0.007). Multiple amputations occurred in 70 patients: 5 quadruple amputations, 27 triple amputations and 38 double amputations. Pelvic fracture occurred in 21 victims, all but one of whom had multiple amputations. Severe perineal, gluteal or genital injuries were present in 46 patients. Severe soft tissue injury was universal, with injection of contaminated soil along tissue planes well above entry sites. There were 13 facial injuries, 9 skull fractures and 3 traumatic brain injuries. Eleven eye injuries were seen; none of the victims with eye injuries were wearing eye protection. The casualty fatality rate was at least 19%. The presence of more than one amputation was associated with a higher rate of pelvic fracture (28.6% vs 3.3%; p=0.005) and perineal–gluteal injury (32.6% vs 11.1%; p=0.009). Conclusion The injury pattern suffered by the target of the AP-IED is markedly worse than that of conventional APM. Pelvic binders and tourniquets should be applied at the point of injury to patients with multiple amputations or perineal injuries